PI组长：
       王旭  长聘教轨副教授

 

研究方向：
       植物线粒体应激机制

 

主持项目：

2022-2025 国家自然科学基金委国际合作重点项目

2021-2024 国家自然科学基金委面上项目

2020-2023 上海市科委面上项目

2019-2022 上海市浦江人才

 

主要研究领域：

以马铃薯、拟南芥为主要实验材料，运用分子生物学、多组学、系统生物学等方法，解析与环境具有密切相互作用的复杂表型，特别是与线粒体密切相关的抗逆、衰老和发育等相关表型。前期研究工作第一次揭示了线粒体未折叠蛋白反应 (mitochondrial unfolded protein response, UPR^mt) 调节植物细胞适应线粒体逆境的过程及调节机制。

目前实验室综合应用系统生物学、细胞生物学、遗传学等手段研究线粒体与细胞核及其他细胞器互作机制，重点关注以下几个主要方向：

 (1) 植物发育及逆境中的线粒体逆向信号

 (2) 线粒体来源信号调节个体与细胞衰老的机制

 (3) 系统生物学在植物线粒体和细胞代谢研究中的应用

 

主要论文：

# 共同第一作者，*共同通讯作者

1. Qi Z#*, Guo C#, Li H#, Qiu H#, Li H, Jong C, Yu G, Zhang Y, Hu L, Wu X, Xin D, Yang M, Liu C, Lv J, Wang X*, Kong F* and Chen Q*: Natural variation in Fatty Acid 9 is a determinant of fatty acid and protein content. Plant Biotechnology Journal 2023, doi.org: 10.1111/pbi.14222.
2. Qu L, Liu M, Zheng L, Wang X, Xue H: Data-independent acquisition-based global phosphoproteomics reveal the diverse roles of casein kinase 1 in plant development. Science Bulletin 2023, 68:2077-2093.
3. Sleiman MB, Jha P, Houtkooper R, Williams R, Wang X*, Auwerx J*. The gene regulatory footprint of aging highlights conserved central regulators. Cell Reports. 2020: 32(13):108203.
4. Oppi S, Nusser-Stein S, Blyszczuk P, Wang X, Jomard A, Marzolla V, Yang K, Velagapudi S, Ward LJ, Yuan XM, Geiger MA, Guillaumon AT, Othman A, Hornemann T, Rancic Z, Ryu D, Oosterveer MH, Osto E, Lüscher TF, Stein S. Macrophage NCOR1 protects from atherosclerosis by repressing a pro-atherogenic PPARγ signature. European Heart Journal. 2020; 41: 995-1005.
5. Houzelle A, Dahlmans D, Nascimento EBM, Schaart G, Jörgensen JA, Moonen-Kornips E, Kersten S, Wang X, Hoeks J. MicroRNA-204-5p modulates mitochondrial biogenesis in C2C12 myotubes and associates with oxidative capacity in humans. Journal of Cellular Physiology. 2020: 1-13.
6. Dahlmans D, Houzelle A, Andreux P, Wang X, Jorgensen JA, Moullan N, Daemen S, Kersten S, Auwerx J, Hoeks J. MicroRNA-382 silencing induces a mitonuclear protein imbalance and activates the mitochondrial unfolded protein response in muscle cells. Journal of Cellular Physiology 2019; 234: 6601-6610.
7. Li H^#, Wang X^#, Rukina D, Huang Q, Lin T, Sorrentino V, Zhang H, Sleiman MB, Arends D, McDaid A, Luan P, Ziari N, Velázquez-Villegas LA, Gariani K, Kutalik Z, Schoonjans K, Radcliffe RA, Prins P, Morgenthaler S, Williams RW, Auwerx J. An integrated systems genetics and omics toolkit to probe gene function. Cell Systems 2018; 6: 90-102.
8. Wang X & Auwerx J. Systems phytohormone responses to mitochondrial proteotoxic stress. Molecular Cell 2017; 68: 540-51.
9. Stein S^#, Lemos V^#, Xu P, Demagny H, Wang X, Ryu D, Jimenez V, Bosch F, Lüscher TF, Oosterveer MH, Schoonjans K. Impaired SUMOylation of nuclear receptor LRH-1 promotes nonalcoholic fatty liver disease. Journal of Clinical Investigation. 2017; 127(2):583-592.
10. Greggio C^#, Jha P^#, Kulkarni SS^#, Lagarrigue S, Broskey NT, Boutant M, Wang X, Conde Alonso S, Ofori E, Auwerx J*, Cantó C*, Amati F*. Enhanced respiratory chain supercomplex formation in response to exercise in human skeletal muscle. Cell Metabolism. 2017; 25(2):301-311.
11. Dahlmans D, Houzelle A, Andreux P, Jörgensen J A., Wang X, de Windt L J., Schrauwen P, Auwerx J, Hoeks J. An unbiased silencing screen in muscle cells identifies miR-320a, miR-150, miR-196b, and miR-34c as regulators of skeletal muscle mitochondrial metabolism. Molecular Metabolism. 2017; 6(11):1429-1442.
12. Luo J, Wang X, Feng L, Li Y, He JX. The mitogen-activated protein kinase kinase 9 (MKK9) modulates nitrogen acquisition and anthocyanin accumulation under nitrogen-limiting condition in Arabidopsis. Biochem Biophys Res Commun. 2017; 487(3):539-544.
13. Zhang H, Ryu D, Wu Y, Gariani K, Wang X, Luan P, D'Amico D, Ropelle ER, Lutolf MP, Aebersold R, Schoonjans K, Menzies KJ*, Auwerx J*. NAD⁺ repletion improves mitochondrial and stem cell function and enhances life span in mice. Science. 2016; 352(6292):1436-43.
14. Ryu D^#, Zhang H^#, Ropelle ER^#, Sorrentino V, Mázala DA, Mouchiroud L, Marshall PL, Campbell MD, Ali AS, Knowels GM, Bellemin S, Iyer SR, Wang X, Gariani K, Sauve AA, Cantó C, Conley KE, Walter L, Lovering RM, Chin ER, Jasmin BJ, Marcinek DJ, Menzies KJ*, Auwerx J*. NAD+ repletion improves muscle function in muscular dystrophy and counters global PARylation. Science Translational Medicine. 2016; 8(361):361ra139.
15. Gariani K^#, Menzies KJ^#, Ryu D, Wegner CJ, Wang X, Ropelle ER, Moullan N, Zhang H, Perino A, Lemos V, Kim B, Park YK, Piersigilli A, Pham TX, Yang Y, Ku CS, Koo SI, Fomitchova A, Cantó C, Schoonjans K, Sauve AA, Lee JY, Auwerx J. Eliciting the mitochondrial unfolded protein response by nicotinamide adenine dinucleotide repletion reverses fatty liver disease in mice. Hepatology. 2016; 63(4):1190-204.
16. Xu P, Oosterveer MH, Stein S, Demagny H, Ryu D, Moullan N, Wang X, Can E, Zamboni N, Comment A, Auwerx J, Schoonjans K. LRH-1-dependent programming of mitochondrial glutamine processing drives liver cancer. Genes & Development. 2016; 30(11):1255-60.
17. Jha P, Wang X, Auwerx J. Analysis of mitochondrial respiratory chain supercomplexes using blue native polyacrylamide gel electrophoresis (BN-PAGE). Curr Protoc Mouse Biol. 2016 Mar 1;6(1):1-14.
18. Wang X^#, Ryu D^#, Houtkooper RH*, Auwerx J*. Antibiotic use and abuse: a threat to mitochondria and chloroplasts with impact on research, health, and environment. BioEssays 2015; 37:1045-53.
19. Moullan N^#, Mouchiroud L^#, Wang X, Ryu D, Williams EG, Mottis A, Jovaisaite V, Frochaux MV, Quiros PM, Deplancke B, Houtkooper RH*, Auwerx J*. Tetracyclines disturb mitochondrial function across eukaryotic models: a call for caution in biomedical research. Cell Reports. 2015; 10(10): 1681–91.
20. Jo YS^#, Ryu D^#, Maida A, Wang X, Evans RM, Schoonjans K, Auwerx J. Phosphorylation of the nuclear receptor corepressor 1 by protein kinase B switches its corepressor targets in the liver in mice. Hepatology. 2015; 62(5):1606-18.
21. Stein S, Oosterveer MH, Mataki C, Xu P, Lemos V, Havinga R, Dittner C, Ryu D, Menzies KJ, Wang X, Perino A, Houten SM, Melchior F, Schoonjans K. SUMOylation-dependent LRH-1/PROX1 interaction promotes atherosclerosis by decreasing hepatic reverse cholesterol transport. Cell Metabolism. 2014; 20(4):603-13.
22. Andreux PA, Mouchiroud L, Wang X, Jovaisaite V, Mottis A, Bichet S, Moullan N, Houtkooper RH, Auwerx J. A method to identify and validate mitochondrial modulators using mammalian cells and the worm C. elegans. Scientific Reports. 2014; 4:5285.
23. Wang YD^#, Wang X^#, Wong YS. Generation of selenium-enriched rice with enhanced grain yield, selenium content and bioavailability through fertilization with selenite. Food Chemistry 2013; 141:2385-93.
24. Wang YD^#, Wang X^#, Ngai SM, Wong YS. Comparative proteomics analysis of selenium responses in selenium-enriched rice grains. Journal of Proteome Research 2013; 12:808-20.
25. Wang X^#, Bian Y^#, Cheng K, Gu LF, Ye M, Zou H, Sun SSM, He JX. A Large-scale protein phosphorylation analysis reveals novel phosphorylation motifs and phosphoregulatory networks in Arabidopsis. Journal of Proteomics 2013; 78:486-98.
26. Wang X^#, Bian Y, Cheng K, Zou H, Sun SSM, He JX. A comprehensive differential proteomic study of nitrate deprivation in Arabidopsis reveals complex regulatory networks of plant nitrogen responses. Journal of Proteome Research 2012; 11:2301-15.
27. Wang YD^#, Wang X^#, Wong YS. Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice. Journal of Proteomics 2012; 75:1849-1866.
28. Zhang JH, Sun LW, Liu LL, Lian J, An SL, Wang X, Zhang J, Jin JL, Li SY, Xi JH. Proteomic analysis of interactions between the generalist herbivore Spodoptera exigua (Lepidoptera: Noctuidae) and Arabidopsis thaliana. Plant Mol Biol Rep. 2010; 28(2):324-333.
29. Li SY, Fan J, Xi JH, Zhang HY, Du HW, Zhou X, Wang X, Ning B, Liu LY, Hao DY. Spleen lymphocyte proteome analysis in bronchial asthma of Wistar rats. Chem J Chinese U. 2007; 28(9):1696-1700.
30. Xi J, Wang X, Li S, Zhou X, Yue L, Fan J, Hao D. Polyethylene glycol fractionation improved detection of low-abundant proteins in two-dimensional electrophoresis analysis of plant proteome. Phytochemistry 2006, 67:2341-2348.

 

招生招聘：

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